Techniques and Equipment

(more details coming soon)

Functional Testing

Physical Function

• Rotarod (overall motor function)

• Treadmill (endurance, aerobic exercise capacity)

• Grip Meter (limb strength)

• Inverted Cling (full body strength/endurance)

• Voluntary Wheel Running, VWR (volitional exercise and activity rate)

Cognitive Function

• Open Field (anxiety and exploratory behavior)
• Y-Maze (working memory)
• Novel Object Recognition (memory)
• Puzzle BOx (executive function)

Contractile Function

• ex vivo / in vitro contractile physiology (force, velocity and power of contraction in soleus, extensor digitorum longus, or diaphragm muscle)

• in vivo contractile physiology (maximum isometric plantar flexor, quadriceps, or dorsiflexor torque output)

Gene Expression

• NGS RNAseq

• rt-qPCR

Protein Expression and Cell Signaling

• Mass Spectrometry

• Western Immunoblotting

Immunohistochemistry

• muscle cell cross-sectional area and fiber typing
• muscle cell culture characterization

Mitochondrial Function

• Citrate Synthase Activity Assay

• High Resolution Respirometry

Gain/Loss of Function

• Viral Vectors

• siRNA

Cell Culture

Immortalized Lines

• C2C12
• MCR5

Primary Cells

• Human Primary Myocytes
• Mouse Primary Myocytes