Techniques and Equipment

(more details coming soon)

Functional Testing

Physical Function

  • Rotarod (overall motor function)

 

  • Treadmill (endurance, aerobic exercise capacity)

 

  • Grip Meter (limb strength)

 

  • Inverted Cling (full body strength/endurance)

 

  • Voluntary Wheel Running, VWR (volitional exercise and activity rate)

 

Contractile Function

  • ex vivo / in vitro contractile physiology

 

  • in vivo contractile physiology

 

Gene Expression

  • NGS RNAseq

 

  • rt-qPCR

 

 

Protein Expression and Cell Signaling

  • Mass Spectrometry

 

  • Western Immunoblotting

Immunohistochemistry

 

 

Mitochondrial Function

  • Citrate Synthase Activity Assay

 

  • High Resolution Respirometry

 

 

Gain/Loss of Function

  • Viral Vectors

 

  • siRNA

 

Cell Culture

Immortalized Lines

  • C2C12
  • MCR5

Primary Cells

  • Human Primary Myocytes
  • Mouse Primary Myocytes